| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Caldanaerobacter subterraneus | TGCGTATTTGGTTGGGCTG | TGCTATAAGCGCAGTTACAGG | 58.74 | 58.18 | 184 | 185 |
100.00%
|
50.00%
|
| Primer 2 | Caldanaerobacter subterraneus | TTGCGTATTTGGTTGGGCTG | TGCTATAAGCGCAGTTACAGG | 59.40 | 58.18 | 185 | 185 |
100.00%
|
50.00%
|
| Primer 3 | Caldanaerobacter subterraneus | ATTGCGTATTTGGTTGGGCTG | TGCTATAAGCGCAGTTACAGG | 59.80 | 58.18 | 186 | 185 |
100.00%
|
50.00%
|
| Primer 4 | Caldanaerobacter subterraneus | CCCCTATGAAGCCAAGGACC | ACAGGGTCGTATCCTCCAGG | 59.82 | 60.40 | 154 | 171 |
100.00%
|
50.00%
|
| Primer 5 | Caldanaerobacter subterraneus | TTCCTGATGCTGGGCTCAC | TCCTGTGGGAGCACAAAGC | 59.70 | 60.23 | 177 | 169 |
100.00%
|
50.00%
|
| Primer 6 | Caldanaerobacter subterraneus | AGTTCCTGATGCTGGGCTC | TCCTGTGGGAGCACAAAGC | 59.39 | 60.23 | 179 | 160 |
100.00%
|
50.00%
|
| Primer 7 | Caldanaerobacter subterraneus | GCGGACAAGCTTATGGTGC | GGTTGCTGCCTTTCCATAAGC | 59.57 | 60.13 | 177 | 157 |
100.00%
|
50.00%
|
| Primer 8 | Caldanaerobacter subterraneus | AAGGCTGTGGAAAGCGAGC | CCTGAGACCACTTCCGCTTC | 60.97 | 60.39 | 153 | 151 |
100.00%
|
50.00%
|
| Primer 9 | Caldanaerobacter subterraneus | TGGTTGTGTGGGCTCTTGG | TCCAATTGCCTCACCCTCC | 60.15 | 59.31 | 158 | 149 |
100.00%
|
50.00%
|
| Primer 10 | Caldanaerobacter subterraneus | CCTGATGCTGGGCTCACAT | TCCTGTGGGAGCACAAAGC | 59.77 | 60.23 | 175 | 149 |
100.00%
|
50.00%
|